Pcmv tag2b pdf download

Welcome to vector database vector database is a digital collection of vector backbones assembled from publications and commercially available sources. Construction of eukaryotic expression vectors of carboxyl terminus and amino terminus of kinase suppressor of ras ksr and their expression in 293t cell line april 2006 zhongguo fei ai za zhi. Joan massagues lab contains the insert lap2 and is published in cancer cell. A a549 cells were cotransfected with plasmid pcmv tag2b n, pcmv tag2b m, pcmv tag2b s, or pcmv tag2b e expressing n, m, s, or e protein, respectively, along with the reporter plasmid pil6luc651 in which the luciferase gene is under the control of il6 promoter. Hepatitis b virus inhibits apolipoprotein a5 expression through its. Arginine methylation by prmt2 controls the functions of the actin. Charles wattss lab contains the insert edd and is published in j biol chem. Johannes hirrlingers lab contains the insert ncreert2 and is published in plos one. Mammalian expression vector for tagging proteins with an nterminal flag epitope.

Search results for pcmv2flag vector at sigmaaldrich. Download fulltext pdf effect of notch signal pathway on h9c2 cardiomyocytes apoptosis induced by hypoxiareoxygenation via rock2 article pdf available in journal of translational medicine 102. For other reading frames, use pcmv tag 2a or pcmv tag 2c. Tagged constructs generated in the pcmv tag vectors can be transfected into mammalian cells and the fusion protein can be easily characterized using commercially. Agilent haloplex custom kits provide unparalleled flexibility to create panels for genomic regions of interest. For other reading frames, use pcmvtag 2a or pcmvtag 2c. All pla cell controls trans fected with empty vectors pcmv. Interaction of promyelocytic leukemiap53 affects signal.

With an efficient library prepfree workflow and excellent amplicon target capture performance, our haloplex custom kits offer superior specificity and coverage. The purified products and the vector pcmvtag2b stratagene. C insert origin cloning sites ecorv 741, xhoi 768 size of insert 5604 bp size of vector 4300 bp concentration 1,288 gl maxi 140109 maureen antibiotics kanr sequencing files remarks. The negative control is parental plasmid pcmvtag2b. Relative luciferase activity was determined by standard. Nucleocapsid protein of sarscov activates interleukin6. Looking for online definition of pcmv or what pcmv stands for. Flagprmt2 and mutants thereof in pcmvtag2b, this paper, na. A target gene inserted into the pcmv tag1 vector can be tagged with the flag epitope nterminal, cterminal, or internal. Pcmv is listed in the worlds largest and most authoritative dictionary database of abbreviations and acronyms the free dictionary. Determination of roles of sarscov n protein in the activation of il6 gene. Molecular cloning and functional characterization of murine toll. Construction of eukaryotic expression vectors of carboxyl. The negative control is parental plasmid pcmv tag2b.